Fate of exogenous recombinant plasmids introduced into mouse and human cells
نویسندگان
چکیده
منابع مشابه
Fate of exogenous recombinant plasmids introduced into mouse and human cells.
We have constructed a number of plasmids selectable in both E. coli and mouse or human cells. Human DNA sequences were inserted and the recombinant plasmids were used to transfect either mouse or human cells by the Ca-phosphate precipitation technique. We have observed that: (i) competent cells uptake large amounts of plasmid DNA; (ii) input plasmids persist in transformed mammalian cells as fr...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1985
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/13.15.5545